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Brazilian Journal of Microbiology
Print version ISSN 1517-8382
On-line version ISSN 1678-4405
KOGA, Paula Célia Mariko et al. Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis. Braz. J. Microbiol. [online]. 2003, vol.34, suppl.1, pp.5-7. ISSN 1517-8382. http://dx.doi.org/10.1590/S1517-83822003000500002.
Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizes the different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detection. Besides, immuno-dot assay seems to be a possible alternative as a capture method.
Keywords : intimin; phenotypical analysis; polyclonal antibody; EPEC; EHEC.