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Brazilian Journal of Microbiology
On-line version ISSN 1678-4405
MENEZES, Caroline Anunciação et al. Capture immunoassay for LT detection produced by enterotoxigenic Escherichia coli in bacterial isolates. Braz. J. Microbiol. [online]. 2003, vol.34, suppl.1, pp.11-13. ISSN 1678-4405. http://dx.doi.org/10.1590/S1517-83822003000500004.
A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has been developed. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture immunoassay is a sensitive tool for detection of E. coli, which produces heat-labile enterotoxin, and is suitable for use in clinical laboratories and epidemiological surveys in developing world.
Keywords : detection; polyclonal antibody; monoclonal antibody; thermo-labile toxin; E. coli.