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Brazilian Journal of Microbiology
On-line version ISSN 1678-4405
SEYFFERT, Núbia; SILVA, Éverton Fagonde da; BERMUDEZ, Viviane Lorenzato and BROD, Claudiomar Soares. Influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospires. Braz. J. Microbiol. [online]. 2003, vol.34, suppl.1, pp.74-75. ISSN 1678-4405. http://dx.doi.org/10.1590/S1517-83822003000500025.
In leptospirosis, the Microscopic Agglutination Test (MAT) is the basis of serological diagnosis and the most widely used. In our study, the MAT was applied to evaluate the influence of temperature, incubation time, antigen density and age of the culture in the agglutinability of Leptospires and the interference of these factors in diagnosis. Three serum samples with titers of 100, 800 and 25.600 to the serovar tande (local isolate) of canicola serogroup were used with different combinations among the four factors. There was a significant relation among all factors, specially between the antigen density and age of the culture.
Keywords : Leptospira; microscopic agglutination; Leptospirosis; laboratorial diagnosis.