SciELO - Scientific Electronic Library Online

 
vol.34 suppl.1Laryngotracheitis: reproducibility of the disease and comparison of diagnostic methodsViral DNA sequences in peripheral blood and vertical transmission of the virus: a discussion about BPV-1 author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Brazilian Journal of Microbiology

On-line version ISSN 1678-4405

Abstract

SEYFFERT, Núbia; SILVA, Éverton Fagonde da; BERMUDEZ, Viviane Lorenzato  and  BROD, Claudiomar Soares. Influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospires. Braz. J. Microbiol. [online]. 2003, vol.34, suppl.1, pp. 74-75. ISSN 1678-4405.  http://dx.doi.org/10.1590/S1517-83822003000500025.

In leptospirosis, the Microscopic Agglutination Test (MAT) is the basis of serological diagnosis and the most widely used. In our study, the MAT was applied to evaluate the influence of temperature, incubation time, antigen density and age of the culture in the agglutinability of Leptospires and the interference of these factors in diagnosis. Three serum samples with titers of 100, 800 and 25.600 to the serovar tande (local isolate) of canicola serogroup were used with different combinations among the four factors. There was a significant relation among all factors, specially between the antigen density and age of the culture.

Keywords : Leptospira; microscopic agglutination; Leptospirosis; laboratorial diagnosis.

        · abstract in Portuguese     · text in English     · English ( pdf epdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License