SciELO - Scientific Electronic Library Online

 
vol.34 suppl.1Viral DNA sequences in peripheral blood and vertical transmission of the virus: a discussion about BPV-1Bovine papillomavirus type 2 in reproductive tract and gametes of slaughtered bovine females author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Brazilian Journal of Microbiology

Print version ISSN 1517-8382

Abstract

CONCEICAO, Fabrício Rochedo et al. LTB-R1: an alternative to swine mycoplasmal pneumoniae control. Braz. J. Microbiol. [online]. 2003, vol.34, suppl.1, pp. 79-81. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822003000500027.

Mycoplasmal pneumoniae is the main respiratory disease in swine. The most efficient way to control it is through the use of vaccines (bacterins), whose production cost is high. The objective of this work was to develop a new alternative for controlling Swine Mycoplasmal Pneumoniae, based on a recombinant subunit vaccine containing the R1 region of P97 adhesin of Mycoplasma hyopneumoniae fused to the B subunit of the heat-labile enterotoxin of Escherichia coli (rLTB-R1). In this work we report the amplification of the genes, genetic fusion between LTB and R1 coding sequences, cloning, construction of the expression vector, as well as expression and purification of rLTB-R1 in E. coli.

Keywords : swine mycoplasmal pneumoniae; P97; LTB.

        · abstract in Portuguese     · text in English     · pdf in English