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Brazilian Journal of Microbiology
On-line version ISSN 1678-4405
LEAL, Maria Betania Batista et al. Influence of initial L-asparagine and glycerol concentrations on the batch growth kinetics of Mycobacterium bovis BCG. Braz. J. Microbiol. [online]. 2004, vol.35, n.4, pp. 337-344. ISSN 1678-4405. http://dx.doi.org/10.1590/S1517-83822004000300013.
The influences of the L-asparagine and glycerol initial concentrations in Sauton medium on the productivities of biomass and colony forming units were studied. The submerged batch cultivations of Mycobacterium bovis were carried out in a 20 L bioreactor. The L-asparagine and glycerol initial concentrations of 4.54 g/L and 25 mL/L, respectively, corresponded to the best biomass productivity, namely 2.5 g/L.day. On the other hand, the concentrations of 2.27 g/L and 25 mL/L, respectively, led to the highest productivity in terms of colony forming units, namely 2.7·106 colonies/mg.day. In addition, by means of the relative consumption analysis of L-asparagine and glycerol (50 and 26% respectively), it was concluded that the concentrations of such components could be reduced, with respect to the original Sauton medium composition, aiming the obtainment of an optimal BCG vaccine production in the bioreactor.
Keywords : BCG; Mycobacterium bovis; culture medium; vaccine; submerged batch cultivation.