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Veterinarian Microbiology • Braz. J. Microbiol. 38 (1) • Mar 2007 • https://doi.org/10.1590/S1517-83822007000100002   copy

Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis

Comparação de um teste in-house e um teste ELISA comercial para diagnóstico de paratuberculose

Authorship SCIMAGO INSTITUTIONS RANKINGS

An in-house PPA-ELISA was compared to a commercial ELISA using a panel of 108 serum samples. In relation to commercial test, in-house assay presented 100% of sensitivity and 83.5% of specificity and a high concordance (kappa> 0.5), which demonstrates that the in-house assay is a valuable tool for use in developing countries.

Cattle; Mycobacterium avium subsp. paratuberculosis; serology

Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br
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