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Industrial Microbiology • Braz. J. Microbiol. 38 (1) • Mar 2007 • https://doi.org/10.1590/S1517-83822007000100013   copy

Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system

Isolamento de Trichosporon spp produtor de lipase e extração enzimática pelo sistema bifásico aquoso

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A lipase-producing yeast strain isolated from crude cheese and identified as Trichosporon spp produced 7.3 U/mL (59.3 U/µg) after 72h of cultivation. Lipase showed optimum activity at pH 7.0-8.0 and 45-50ºC. Extraction by the two-phase aqueous system (PEG-phosphate salts) showed an elevated recuperation (99.8%) of enzymatic activity in the PEG phase.

extracellular lipase; Trichosporon; aqueous two phase system; PEG

Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br
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