SciELO - Scientific Electronic Library Online

vol.38 issue1Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous systemMorphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Brazilian Journal of Microbiology

Print version ISSN 1517-8382On-line version ISSN 1678-4405


MALDONADE, Iriani R.; SCAMPARINI, Adilma R. P.  and  RODRIGUEZ-AMAYA, Délia B.. Selection and characterization of carotenoid-producing yeasts from Campinas region, Brazil. Braz. J. Microbiol. [online]. 2007, vol.38, n.1, pp.65-70. ISSN 1517-8382.

The objective of the present study was to select and identify yeasts from Brazil capable of producing carotenoids. Pigmented yeasts were isolated from soil, leaves, fruits, flowers and a processed product. The samples were incubated at 30ºC in Erlenmeyer flasks, containing YM broth. After 48 hours, they were inoculated in Petri dishes with YM agar, and incubated at 30ºC during 120 hours. The yeast colonies, which presented yellow to red coloration, were transferred to culture tubes containing YM agar, and incubated at 30ºC for 72 hours. Out of 242 samples, only five had yellow to red color at high intensity. These highly pigmented yeasts were re-isolated in Petri dishes with YM agar and then transferred to tubes with GPYM agar. Identification through morphological and reproduction characteristics, along with physiological and biochemical tests, classified four strains as R. mucilaginosa and one strain as R. graminis. The main carotenoids extracted from them were identified through HPLC analysis as b-carotene and torulene. The strains showed potential as promising microorganisms for the commercial production of carotenoids.

Keywords : pigmented yeasts; identification; Rhodotorula; carotenoid; fermentation.

        · abstract in Portuguese     · text in English     · English ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License