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Brazilian Journal of Microbiology

Print version ISSN 1517-8382

Abstract

CUNHA-SANTINO, Marcela B. da  and  BIANCHINI JR., Irineu. Cellulase activities during decomposition of a submerged aquatic macrophyte (Utricularia breviscapa): a microcosm assay. Braz. J. Microbiol. [online]. 2007, vol.38, n.2, pp. 230-236. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822007000200008.

Decomposition of lignocellulosic detritus at enzymatic level is the rate-limiting step during aquatic macrophyte decomposition. Assays were carried out to evaluate cellulase activity during decomposition of Utricularia breviscapa; the assays included the following C-sources: leachate, lignocellulosic matrix and integral detritus. The incubations comprised U. breviscapa C-sources added to Óleo lagoon (21º 36'S and 47º 49'W) water maintained in the dark under anaerobic condition at 15ºC, 20ºC, 25ºC and 30ºC. Cellulase activity were monitored during 180 days, with the activity showing a time evolution that depended on the temperature and type of detritus: (i) cellulase activity tended to occur earlier with increasing temperatures, but it was less intense than at lower temperatures, (ii) no activity was observed in incubations with leachate, (iii) cellulase activity was observed in the incubation with integral detritus and lignocellulosic matrix and (iv) higher cellulase activity was observed in the incubations with integral detritus at 15ºC (P < 0.05).

Keywords : cellulase; Utricularia breviscapa; cellulose.

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