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Brazilian Journal of Microbiology

Print version ISSN 1517-8382

Abstract

ANDRADE, Luciana et al. Distribution of HNA and LNA bacterial groups in the Southwest Atlantic Ocean. Braz. J. Microbiol. [online]. 2007, vol.38, n.2, pp. 330-336. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822007000200028.

Bacterioplankton was studied in a large area of Southwest Atlantic Ocean between 13 and 25ºS and 28 and 42ºW. Samples were collected in 108 stations at 20 m depth. Bacteria were enumerated by flow cytometry after nucleic acid staining with syto13 and two subgroups were differentiated: low nucleic acid content (LNA) and high nucleic acid content (HNA) bacteria. Total bacterial numbers varied from 0.37 to 5.53 105 cells mL-1. HNA cells represented 15 to 70% of the total number while LNA cells represented 30 to 85%. Heterotrophic bacterial production was determined by incorporation of tritiated leucine and ranged from 2.7 to 171.07 ng C L-1 h-1. No significant correlation was found between abundance and production. Nevertheless with support of multivariate analysis between bacterial abundance, bacterial production, chlorophyll a and other oceanographic data the distribution of the groups in two different oceanic provinces could be explained by nutrient availability. HNA bacteria accounted for the high percentage of cells found in the area north of 19ºS, linked to higher temperature waters and riverine nutrients inputs. LNA bacteria were the dominant cells south of this latitude and were correlated to the higher values of nitrate found for the same area.

Keywords : bacterial abundance; HNA bacteria; LNA bacteria; flow cytometry; Southwest Atlantic Ocean.

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