SciELO - Scientific Electronic Library Online

 
vol.38 issue3Do DNA extraction methods and Taq polimerase quality improve the double repetitive element (DRE) PCR typing method for Mycobacterium tuberculosis strains?An outbreak of Streptococcus dysgalactiae subsp equisimilis in a hospital in the south of Brazil author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Brazilian Journal of Microbiology

Print version ISSN 1517-8382On-line version ISSN 1678-4405

Abstract

CAMPOS, Angélica Cristine de Almeida et al. Comparison between ectodomain and G2 region of G glycoprotein for genotyping of HRSV. Braz. J. Microbiol. [online]. 2007, vol.38, n.3, pp.413-416. ISSN 1517-8382.  https://doi.org/10.1590/S1517-83822007000300005.

The Human Respiratory Syncytial Virus (HRSV), isolated in 1955, is the main cause of hospitalization of babies and infants with respiratory illness. Several studies have been conducted worldwide aiming the development of a safe and effective vaccine against HRSV. The G2 region of glycoprotein G is used as genotyping default. In the present study, we performed a phylogenetic analysis of G protein and a comparative study between G2 region and ectodomain of attachment glycoprotein. Fifty-three nasal swab samples from children less than 5 years old and presenting symptoms of acute respiratory illness, assisted at the University Hospital (UH) of University of Sao Paulo (USP) in 2004, were submitted to sequencing by PCR and compared with GenBank sequences. We concluded that the G2 region is adequate for HRSV genotyping.

Keywords : Human Respiratory Syncytial Virus; G Glycoprotein; G2 Region; Phylogenetic Analysis.

        · abstract in Portuguese     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License