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Brazilian Journal of Microbiology
On-line version ISSN 1678-4405
GOMES, Bruna C. et al. Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foods. Braz. J. Microbiol. [online]. 2007, vol.38, n.4, pp. 617-619. ISSN 1678-4405. http://dx.doi.org/10.1590/S1517-83822007000400007.
We evaluated the suitability of API 20 STREP and multiplex PCR to speciate 52 Enterococcus spp. obtained from Brazilian foods. A high percentage of isolates (78.9%) presented discrepant results between evaluated tests. Similar results were obtained for six E. faecalis and five E. faecium. The PCR multiplex was more effective than API 20 STREP for complete identification of the isolates.
Keywords : Enterococcus spp.; identification; API 20 STREP; PCR multiplex; foods.