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Brazilian Journal of Microbiology

Print version ISSN 1517-8382

Abstract

CAFFARO-FILHO, Roberto A.; FANTINATTI-GARBOGGINI, Fabiana  and  DURRANT, Lucia R.. Quantitative analysis of Terminal Restriction Fragment Length Polymorphism (T-RFLP) microbial community profiles: peak height data showed to be more reproducible than peak area. Braz. J. Microbiol. [online]. 2007, vol.38, n.4, pp. 736-738. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822007000400027.

Terminal Restriction Fragment Length Polymorphism (T-RFLP) is a culture-independent fingerprinting method for microbial community analysis. Profiles generated by an automated electrophoresis system can be analysed quantitatively using either peak height or peak area data. Statistical testing demontrated that peak height data showed to be more reproducible than peak area data.

Keywords : Terminal Restriction Fragment Length Polymorphism (T-RFLP); Molecular analysis; Microbial community.

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