Brazilian Journal of Microbiology
On-line version ISSN 1678-4405
GOES, Luiz Gustavo Bentim et al. Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression. Braz. J. Microbiol. [online]. 2008, vol.39, n.1, pp. 1-4. ISSN 1678-4405. http://dx.doi.org/10.1590/S1517-83822008000100001.
The development of a bovine papillomavirus (BPV) vaccine is an outstanding challenge. BPV protein L1 gene transfection in the Drosophila melanogaster S2 cell expression system failed to produce L1 protein notwithstanding correct L1 gene insertion. Severe genetic inbalance in the host cell line, including cytogenetic alterations, may account for the lack of protein expression.
Keywords : Bovine Papillomavirus; BPV4; S2 expression cells.