Brazilian Journal of Microbiology
versão On-line ISSN 1678-4405
PIO, Tatiana Fontes; FRAGA, Laira Priscila e MACEDO, Gabriela Alves. Inoculum padronization for the production of cutinase by Fusarium oxysporum. Braz. J. Microbiol. [online]. 2008, vol.39, n.1, pp. 74-77. ISSN 1678-4405. http://dx.doi.org/10.1590/S1517-83822008000100018.
Cutinase is a versatile enzyme showing several interesting properties for application in industrial processes. The widespread use of this enzyme depends on the development of an efficient and low-cost production system. One of the most important steps in a fermentation process is the standardization of the inoculum characteristics. In this study, the production of cutinase by Fusarium oxysporum showed a statistically significant relationship with both the inoculum size and the inoculum PDA pH. The greatest activities were 19.1 U/mL at PDA pH 7.0 and 22.72 U/mL using an aliquot of 12.72 x 107 spores/mL. The macroscopic characteristics of the colonies of Fusarium oxysporum changed according to the variation of the medium pH, with the best results recorded in those colonies presenting a cotton white aspect.
Palavras-chave : Fusarium oxysporum; inoculum; cutinase; macroscopic characteristics; fermentation process.