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vol.39 número1Produção e caracterização da glucoamilase do fungo Aspergillus awamori expressa em levedura Saccharomyces cerevisiae usando diferentes fontes de carbonoProduction of endoglucanase by the native strains of Streptomyces isolates in submerged fermentation índice de autoresíndice de assuntospesquisa de artigos
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Brazilian Journal of Microbiology

versão impressa ISSN 1517-8382versão On-line ISSN 1678-4405

Resumo

JACOB, Nicemol  e  PREMA, Parukuttyamma. Novel process for the simultaneous extraction and degumming of banana fibers under solid-state cultivation. Braz. J. Microbiol. [online]. 2008, vol.39, n.1, pp.115-121. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822008000100025.

Various process parameters for the production of polygalacturonase by Streptomyces lydicus under solid-state fermentation were optimized. The optimum particle size of wheat bran for polygalacturonase production was in the range of 500-1000 µm. Initial moisture content of 70% was found to be the optimum for enzyme production. The most suitable inoculum size was 1.25 x 105 CFU/mL and the optimum incubation temperature was 30ºC. Addition of carbon sources resulted in 37% increase in enzyme yield (425 U/g), whereas no significant enhancement was obtained on nitrogen supplementation. Maximum enzyme yield was recorded at 72 h. When compared to the initial production medium (108.5 U/g), the enzyme yield was 3.9 fold after optimization. Solid-state fermentation was effectively employed to develop a novel process for the simultaneous extraction and degumming of banana fibers. Streptomyces lydicus was allowed to grow on wheat bran medium in which banana leaf sheath pieces were incorporated and the fiber bundles were separated after a two-step fermentative process.

Palavras-chave : Banana fiber degumming; Solid-state fermentation; Polygalacturonase; Streptomyces lydicus; Wheat bran.

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