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Brazilian Journal of Microbiology

versão impressa ISSN 1517-8382versão On-line ISSN 1678-4405

Resumo

CHELLAPANDI, P.  e  JANI, Himanshu M.. Production of endoglucanase by the native strains of Streptomyces isolates in submerged fermentationProdução de endoglucanase por cepas nativas de Streptomyces em fermentação submersa. Braz. J. Microbiol. [online]. 2008, vol.39, n.1, pp.122-127. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822008000100026.

Cellulase is a complex enzyme system, commercially produced by filamentous fungi under solid-state and submerged cultivation. It has wide applicability in textile, food and beverage industry for effective saccharification process. In this study, cellulolytic enzyme activity, particularly endoglucanase of 26 Streptomyces strains isolated from garden soil was examined, including two isolates selected on the basis of potential cellulolytic activity on Bennett's agar medium. To enhance the endoglucanase formation in broth culture, different conditions including carbon and nitrogen sources, and growth conditions were tested. The maximum endoglucanase activity (11.25-11.90 U/mL) was achieved within 72-88 h in fermentation medium containing Tween-80, followed by phosphate sources. Both cellulolytic Streptomyces isolates gave almost equal quantity of enzyme in all trials. However the effect of medium ingredients on endoglucanase induction diverged with strains in some extent.

Palavras-chave : endoglucanase; Streptomyces isolates; Submerged cultivation; Media optimization; Cellulase; CMCase.

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