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Brazilian Journal of Microbiology

Print version ISSN 1517-8382On-line version ISSN 1678-4405

Abstract

AMBROSIO, Simone Rodrigues et al. Comparison of three decontamination methods for Mycobacterium bovis isolation. Braz. J. Microbiol. [online]. 2008, vol.39, n.2, pp.241-244. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822008000200008.

Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method.

Keywords : Mycobacterium bovis; decontamination; Petroff; HPC.

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