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Brazilian Journal of Microbiology

versão impressa ISSN 1517-8382versão On-line ISSN 1678-4405

Resumo

MATROUDI, S.; ZAMANI, M.R.  e  MOTALLEBI, M.. Molecular cloning of chitinase 33 (chit33) gene from Trichoderma atroviride. Braz. J. Microbiol. [online]. 2008, vol.39, n.3, pp.433-437. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822008000300005.

In this study Trichoderma atroviride was selected as over producer of chitinase enzyme among 30 different isolates of Trichoderma sp. on the basis of chitinase specific activity. From this isolate the genomic and cDNA clones encoding chit33 have been isolated and sequenced. Comparison of genomic and cDNA sequences for defining gene structure indicates that this gene contains three short introns and also an open reading frame coding for a protein of 321 amino acids. The deduced amino acid sequence includes a 19 aa putative signal peptide. Homology between this sequence and other reported Trichoderma Chit33 proteins are discussed. The coding sequence of chit33 gene was cloned in pEt26b(+) expression vector and expressed in E. coli.

Palavras-chave : Trichoderma atroviride; chit33; chitinase activity; gene structure.

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