SciELO - Scientific Electronic Library Online

vol.39 issue3Comparison of agglutinating and neutralizing antibodies to serovar hardjo in sows immunized with two commercial whole culture polivalent anti-leptospira bacterinsMolecular genetic differentiation of avian Escherichia coli by RAPD-PCR author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Brazilian Journal of Microbiology

Print version ISSN 1517-8382On-line version ISSN 1678-4405


AGUIAR, Daniel M.; HAGIWARA, Mitika K.  and  LABRUNA, Marcelo B.. In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil. Braz. J. Microbiol. [online]. 2008, vol.39, n.3, pp.489-493. ISSN 1517-8382.

An Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains, respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28 partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is the only recognized Ehrlichia species infecting dogs in Brazil.

Keywords : Ehrlichia canis; Dog; Molecular Characterization; Ehrlichiosis.

        · abstract in Portuguese     · text in English     · English ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License