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Brazilian Journal of Microbiology

versão impressa ISSN 1517-8382versão On-line ISSN 1678-4405

Resumo

RAMOS, Aline de Souza; FIAUX, Sorele Batista  e  LEITE, Selma Gomes Ferreira. Production of 6-pentyl-α-pyrone by trichoderma harzianum in solid-state fermentation. Braz. J. Microbiol. [online]. 2008, vol.39, n.4, pp.712-717. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822008000400022.

Many Trichoderma species are able to produce 6-pentyl-α-pyrone (6-PP), a lactone with coconut-like aroma. In the present work, several culture parameters were studied to enhance the production of 6-PP by Trichoderma harzianum 4040 in solid-state fermentation. Green coir powder added to a nutrient solution was used as support material for fermentation. A Plackett-Burman screening technique was applied, followed by a fractionary factorial design. The best culture conditions within the experimental domain studied were (100 g support)-1: sucrose, 3 g; NaNO3, 0.24 g; (NH4)2SO4, 0.18 g; KH2PO4, 0.1 g; inoculum concentration, 2.2 x 106 spores; moisture level, 55%. The temperature established was 28ºC. The fermentation under the selected conditions led to a 6-PP production six times higher (5.0 mg/g dry matter) than the initial one (0.8 mg/g dry matter) after seven days of cultivation.

Palavras-chave : 6-pentyl-α-pyrone; solid-state fermentation; experimental design.

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