Brazilian Journal of Microbiology
On-line version ISSN 1678-4405
FIGUEIREDO, Eduardo Eustáquio de Souza et al. Identification of Mycobacterium bovis Isolates by a multiplex PCR. Braz. J. Microbiol. [online]. 2009, vol.40, n.2, pp. 231-233. ISSN 1678-4405. http://dx.doi.org/10.1590/S1517-83822009000200004.
Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.
Keywords : Mycobacterium bovis; multiplex-PCR; bovine tuberculosis.