SciELO - Scientific Electronic Library Online

 
vol.40 issue2Identification of Mycobacterium bovis Isolates by a multiplex PCRMolecular analysis of the rRNA genes of Babesia spp and Ehrlichia canis detected in dogs from Ribeirão Preto, Brazil author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Brazilian Journal of Microbiology

Print version ISSN 1517-8382

Abstract

BIANCHINI, A.A.C. et al. Activation of the alternative complement pathway in canine normal serum by Paracoccidioides brasiliensis. Braz. J. Microbiol. [online]. 2009, vol.40, n.2, pp. 234-237. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822009000200005.

The dimorphic fungus Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, a human granulomatous disease. Recently the first case of natural disease in dogs was reported. The complement system is an important effector component of humoral immunity against infectious agents. Therefore, the aim of this study was to evaluate the activation of the dog alternative complement pathway by P. brasiliensis. Initially, the ability of erythrocytes of guinea pig, rabbit, sheep, chicken and swine to activate the dog alternative pathway was evaluated. The guinea pig erythrocytes showed the greatest capacity to activate dog alternative pathway. The alternative (AH50) hemolytic activity was evaluated in 27 serum samples from healthy dogs and the mean values were 87.2 AH50/ml. No significant differences were observed in relation to sex and age. The alternative pathway activation by P. brasiliensis was higher in serum samples from adult dogs when compared to puppies and aged dogs (p < 0.05). This is the first report of dog alternative complement pathway activation by P. brasiliensis and suggests that it may play a protective role in canine paracoccidioidomycosis.

Keywords : Paracoccidioidomycosis; dog; complement.

        · abstract in Portuguese     · text in English     · pdf in English