SciELO - Scientific Electronic Library Online

 
vol.40 issue2Genetic diversity of siderophore-producing bacteria of tobacco rhizospherePtSRR1, a putative Pisolithus tinctorius symbiosis related receptor gene is expressed during the first hours of mycorrhizal interaction with Castanea sativa roots author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Brazilian Journal of Microbiology

Print version ISSN 1517-8382

Abstract

URSZULA, Guzik; IZABELA, Greń; DANUTA, Wojcieszyńska  and  SYLWIA, Łabużek. Isolation and characterization of a novel strain of Stenotrophomonas maltophilia possessing various dioxygenases for monocyclic hydrocarbon degradation. Braz. J. Microbiol. [online]. 2009, vol.40, n.2, pp. 285-291. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822009000200014.

A Gram-negative bacterium, designated as strain KB2, was isolated from activated sludge and was found to utilize different aromatic substrates as sole carbon and energy source. On the basis of morphological and physiochemical characteristics and 16S rRNA gene sequence analysis, the isolated strain KB2 was identified as Stenotrophomonas maltophilia. Strain KB2 is from among different Stenotrophomonas maltophilia strains the first one described as exhibiting the activities of three types of dioxygenases depending on the structure of the inducer. The cells grown on benzoate and catechol showed mainly catechol 1,2- dioxygenase activity. The activity of 2,3-dioxygenase was detected after phenol induction. Protocatechuate 3,4-dioxygenase was found in crude cell extracts of this strain after incubation with 4-hydroxybenzoic acid, protocatechuic acid and vanillic acid. Because of broad spectrum of dioxygenases' types that Stenotrophomonas maltophilia KB2 can exhibit, this strain appears to be very powerful and useful tool in the biotreatment of wastewaters and in soil decontamination.

Keywords : biodegradation; Stenotrophomonas; aromatic compounds; dioxygenases.

        · abstract in Portuguese     · text in English     · pdf in English