SciELO - Scientific Electronic Library Online

 
vol.40 issue2Spore production in Paecilomyces lilacinus (Thom.) samson strains on agro-industrial residuesFungicide resistance and genetic variability in plant pathogenic strains of Guignardia citricarpa author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Brazilian Journal of Microbiology

Print version ISSN 1517-8382

Abstract

RODRIGUES, Andréa dos Santos; VALDMAN, Belkis  and  SALGADO, Andréa Medeiros. Analysis of methane biodegradation by Methylosinus trichosporium OB3b. Braz. J. Microbiol. [online]. 2009, vol.40, n.2, pp. 301-307. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822009000200017.

The microbial oxidation of methane in the atmosphere is performed by methanotrophic bacteria that use methane as a unique source of carbon and energy. The objective of this work consisted of the investigation of the best conditions of methane biodegradation by methanotrophic bacteria Methylosinus trichosporium OB3b that oxidize it to carbon dioxide, and the use of these microorganisms in monitoring methods for methane. The results showed that M. trichosporium OB3b was capable to degrade methane in a more effective way with an initial microorganism concentration of 0.0700 g.L-1, temperature of 30ºC, pH 6.5 and using 1.79 mmol of methane. In these same conditions, there was no bacterial growth when 2.69 mmol of methane was used. The specific rate of microorganism growth, the conversion factor, the efficiency and the volumetric productivity, for the optimized conditions of biodegradation were, respectively, 0.0324 h-1, 0.6830 gcells/gCH4, 73.73% and 2.7732.10-3 gcells/L.h. The final product of methane microbiological degradation, carbon dioxide, was quantified through the use of a commercial electrode, and, through this, the grade of methane conversion in carbon dioxide was calculated.

Keywords : methane; biodegradation; methanotrophic bacteria; Methylosinus trichosporium OB3b.

        · abstract in Portuguese     · text in English     · pdf in English