Brazilian Journal of Microbiology
Print version ISSN 1517-8382
On-line version ISSN 1678-4405
RAMAZANZADEH, Rashid; FARNIA, Parisa and AMIRMOZAFARI, Nour. Characterization of Mycobacterium tuberculosis complex isolated from iranian and afghani patients by spoligotyping method. Braz. J. Microbiol. [online]. 2009, vol.40, n.2, pp.314-320. ISSN 1517-8382. http://dx.doi.org/10.1590/S1517-83822009000200019.
Designing newer drugs, vaccines, and diagnostic techniques is dependent on better understanding of M. tuberculosis virulence mechanism. In this study the prevalence of pcaA gene was determined in M. tuberculosis strains typed by spoligotyping. The associated risk factors among patients with different nationalities residing in Iran were also determined. The isolated M. tuberculosis strains have been characterized by performing susceptibility tests against four first-line antituberculosis drugs and were then subjected to spoligotyping characterization. PCR was used for detection of pcaA gene and its nucleotide sequence was also determined. Spoligotyping of M. tuberculosis strains resulted in 140 different patterns. One hundred twenty two (87.1%) of these spoligotype isolates were unique and reported for the first time. The remaining18 (12.8%) spoligotype patterns were previously reported from other geographical regions of the world. Haarlem family was most prevalent than other genotype. Antibiotic resistances were higher in those isolated from the Iranian patients. The pcaA gene was detected in M. tuberculosis clinical isolates but not in saprophyte strains such as M. kansasi. The results showed that, spread of M. tuberculosis strains belonging to the Beijing family among Iranian patients has to be considered seriously. This study confirmed the widespread existenceof pcaA gene in almost all the clinical isolates. It is also important to undertake studiesto identify which factors are the most significant to considerin tuberculosis control program.
Keywords : tuberculosis; resistance; drugs; spoligotyping; pcaA.