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Brazilian Journal of Microbiology

Print version ISSN 1517-8382On-line version ISSN 1678-4405


MILAGRE, H.M.S.; MARTINS, L.R.  and  TAKAHASHI, J.A.. Novel agents for enzymatic and fungal hydrolysis of stevioside. Braz. J. Microbiol. [online]. 2009, vol.40, n.2, pp.367-372. ISSN 1517-8382.

A comparative study on the potential of some biological agents to perform the hydrolysis of stevioside was carried out, aiming at establishing an alternative methodology to achieve the aglycon steviol or its rearranged derivative isosteviol, in high yields to be used in the preparation of novel bioactive compounds. Hydrolysis reactions were performed by using filamentous fungi (Aspergillus niger, Rhizopus stolonifer and Rhizopus arrhizus), a yeast (Saccharomyces cerevisiae)andenzymes (pancreatin and lipases PL250 and VFL 8000). Pancreatin showed the best hydrolytic activity, furnishing isosteviol at 93.9% of yield, at pH 4.0, using toluene as a co-solvent. Steviol was produced using both pancreatin at pH 7.0 (20.2% yield) and A. niger at pH 7 (20.8% yield).

Keywords : stevioside; hydrolysis; Aspergillus niger; lipase; pancreatin.

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