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Brazilian Journal of Microbiology
On-line version ISSN 1678-4405
BASTOS, Camila V.; PASSOS, Lygia M. F.; VASCONCELOS, Maria Mercês C. and RIBEIRO, Múcio F. B.. In vitro establishment and propagation of a Brazilian strain ofAnaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells. Braz. J. Microbiol. [online]. 2009, vol.40, n.2, pp. 399-403. ISSN 1678-4405. http://dx.doi.org/10.1590/S1517-83822009000200034.
A Brazilian isolate of Anaplasma marginale with appendage was successfully established and maintained in vitro in a tick cell line (IDE8). Infection was confirmed by optical and transmission electron microscopy. In addition, primers MSP1aNF2 and MSP1aNR2 amplified products from DNA extracted from infected IDE8 cells. Comparisons with partial sequences of the msp1α gene and the complete genome of A. marginale confirmed that the sequences of amplified fragments were from the A. marginale genome. This is the first establishment of a Brazilian A. marginale isolate in tick cells, representing a new system for biological and molecular studies and also a new source of material for diagnosis and development of vaccines.
Keywords : IDE8 cells; Anaplasma marginale; in vitro culture.