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Brazilian Journal of Microbiology

Print version ISSN 1517-8382


VIRAMONTES-RAMOS, Sabina et al. Selection of biosurfactan/bioemulsifier-producing bacteria from hydrocarbon-contaminated soil. Braz. J. Microbiol. [online]. 2010, vol.41, n.3, pp.668-675. ISSN 1517-8382.

Petroleum-derived hydrocarbons are among the most persistent soil contaminants, and some hydrocarbon-degrading microorganisms can produce biosurfactants to increase bioavailability and degradation. The aim of this work was to identify biosurfactant-producing bacterial strains isolated from hydrocarbon-contaminated sites, and to evaluate their biosurfactant properties. The drop-collapse method and minimal agar added with a layer of combustoleo were used for screening, and positive strains were grown in liquid medium, and surface tension and emulsification index were determined in cell-free supernantant and cell suspension. A total of 324 bacterial strains were tested, and 17 were positive for the drop-collapse and hydrocarbon-layer agar methods. Most of the strains were Pseudomonas, except for three strains (Acinetobacter, Bacillus, Rhodococcus). Surface tension was similar in cell-free and cell suspension measurements, with values in the range of 58 to 26 (mN/m), and all formed stable emulsions with motor oil (76-93% E24). Considering the variety of molecular structures among microbial biosurfactants, they have different chemical properties that can be exploited commercially, for applications as diverse as bioremediation or degradable detergents.

Keywords : biosurfactants; bioemulsifiers; soil microbiota; hydrocarbon contamination.

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