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Brazilian Journal of Microbiology

versão impressa ISSN 1517-8382


TAO, Nengguo; GAO, Yumei  e  LIU, Yuejin. Isolation and characterization of a Pichia anomala strain: a promising candidate for bioethanol production. Braz. J. Microbiol. [online]. 2011, vol.42, n.2, pp.668-675. ISSN 1517-8382.

A yeast strain designated as Y-1 was isolated and characterized from wine yeast ("Jiuqu"). Based on the morphological and biochemical results, along with the rDNA internal transcribed spacer region (ITS), Y-1 was identified to be a Pichia anomala strain. Y-1 is an ethanol-tolerant strain, enduring ethanol concentrations of up to 14 %. Y-1 growth medium conditions were optimized, results showing good growth in medium with pH ranges from 3.5-6.5, temperature ranges from 25-30 °C, and inoculums range of 8 %-12 %, while optimum growth conditions were reached at a temperature of 30 °C, pH 5.0, and inoculums of 10 %. Furthermore, when the alkaline hydrolyzed Shatian pummelo peel solutions were inoculated with 10 % Y-1 and fermented at 30 °C for 6 d, 4.7 % pure ethanol (w/w) was produced, as evidenced by gas chromatography analysis. Our present study shows potential for the Y-1 strain to be a promising candidate for bioethanol production.

Palavras-chave : Bioethanol; gas chromatography analysis; Jiuqu; Pichia anomala.

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