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Brazilian Journal of Microbiology

versão impressa ISSN 1517-8382

Resumo

LOBO, Evelyn et al. Mycoplasmas hyorhinis in different regions of cuba: diagnosis. Braz. J. Microbiol. [online]. 2011, vol.42, n.2, pp.721-725. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822011000200039.

M. hyorhinis is considered one of the etiological agents of arthritis in sucking pigs, but recently as seen, some strains can produce pneumonia that could not be distinguished from the mycoplasmosis caused by M. hyopneumoniae. The study was conducted to research the presence of Mycoplasma hyorhinis (M. hyorhinis ) in different regions of the country from exudates of pig lungs with typical EP lesions. Exudates from 280 pig lungs with typical EP lesions were studied using molecular techniques such as PCR, real time PCR and amplification of the 16S-23S rRNA. It was detected that the 66% of the samples studied resulted positive to M. hyorhinis, and the presence of this species was detected in all the provinces. Amplification and studies on the intergenic region 16S-23S of M. hyorhinis rRNA demonstrated the existing variability among strains of a same species. This study is the first report on M. hyorhinis detection in Cuba.

Palavras-chave : PCR; mycoplasma; diagnostic; enzootic pneumonia.

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