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Brazilian Journal of Microbiology

versão impressa ISSN 1517-8382

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MANIKANDAN, Muthu; KANNAN, Vijayaraghavan; VELIKONJA, Blagajana Herzog  e  PAšIC, Lejla. Optimization of growth medium for protease production by Haloferax Lucentensis VKMM 007 by response surface methodology. Braz. J. Microbiol. [online]. 2011, vol.42, n.2, pp.818-824. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822011000200050.

The production of halophilic thermostable protease by Haloferax lucentensis VKMM 007 was optimized using a statistical approach. In accordance with factorial design, soluble starch, gelatin, KCl and MgSO4 were selected among 27 variables tested. Next, a second-order quadratic model was estimated and optimal medium concentrations were determined based on quadratic regression equation generated by model. These were 5.14 g L-1 of KCl, 6.57 g L-1of MgSO4, 9.05 g L-1of gelatin and 5.27 g L-1of soluble starch in high salts media supplemented with 0.5% (w/v) of beef extract and peptone, respectively. In these optimal conditions, the obtained protease concentration of 6.80 U mL-1 was in agreement with the predicted protease concentration and was further improved to 7.02 U mL-1 by increasing the concentration of NaCl in the medium to 25% (w/v). An overall 4.0-fold increase in protease production was achieved in the optimized medium compared to activity obtained in initial medium.

Palavras-chave : cultivation media; Haloferax lucentensis; Response surface methodology; optimization; halophilic protease.

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