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Brazilian Journal of Microbiology
Print version ISSN 1517-8382
VAUCHER, Rodrigo de Almeida et al. Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil. Braz. J. Microbiol. [online]. 2011, vol.42, n.4, pp.1440-1444. ISSN 1517-8382. http://dx.doi.org/10.1590/S1517-83822011000400029.
Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.
Keywords : Bovine parainfluenza virus type 3; bPIV-3; phylogenetic analysis.