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Brazilian Journal of Microbiology
Print version ISSN 1517-8382
HAO, Zhikui et al. Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1. Braz. J. Microbiol. [online]. 2012, vol.43, n.1, pp. 177-186. ISSN 1517-8382. http://dx.doi.org/10.1590/S1517-83822012000100019.
The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value.
Keywords : chitinase; medium optimization; response surface method; inulin; Chitinolyticbacter meiyuanensis SYBC-H1.