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Brazilian Journal of Microbiology

Print version ISSN 1517-8382

Abstract

WOLSKA, Katarzyna; KOT, Barbara  and  JAKUBCZAK, Antoni. Phenotypic and genotypic diversity of Pseudomonas aeruginosa strains isolated from hospitals in siedlce (Poland). Braz. J. Microbiol. [online]. 2012, vol.43, n.1, pp. 274-282. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822012000100032.

A total of 62 Pseudomonas aeruginosa strains isolated from two hospitals in Siedlce (Poland) were studied by repetitive element based PCR (rep-PCR) using BOX primer. BOX-PCR results revealed the presence of 7 numerous genotypes and 31 unique patterns among isolates. Generally, the strains of P. aeruginosa were characterized by resistance to many antibiotics tested and by differences in serogroups and types of growth on cetrimide agar medium. However, the P. aeruginosa strains isolated from faeces showed much lower phenotypic and genotypic variations in comparison with strains obtained from other clinical specimens. It was observed that genetic techniques supported by phenotypic tests have enabled to conduct a detailed characterization of P. aeruginosa strains isolated from a particular environment at a particular time.

Keywords : Pseudomonas aeruginosa; BOX-PCR; antibiotic resistance; serotyping; cetrimide agar.

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