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Brazilian Journal of Microbiology
Print version ISSN 1517-8382
NAVARRO-NOYA, Yendi et al. 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting. Braz. J. Microbiol. [online]. 2012, vol.43, n.1, pp. 283-287. ISSN 1517-8382. http://dx.doi.org/10.1590/S1517-83822012000100033.
Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.
Keywords : endophthalmitis; Staphylococcus epidermidis; PCR; DGGE.