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Brazilian Journal of Microbiology
Print version ISSN 1517-8382
RADHAKRISHNAN, Ananthi and ANANTHASUBRAMANIAN, M. Characterization and lytic activity of Pseudomonas fluorescens phages from sewage. Braz. J. Microbiol. [online]. 2012, vol.43, n.1, pp. 356-362. ISSN 1517-8382. http://dx.doi.org/10.1590/S1517-83822012000100042.
Pseudomonas fluorescens phages from sewage were tested against P. fluorescens isolates of soil and sewage. The phages were characterized as to host range, morphology, structural proteins and genome fingerprint. Of the seven phages isolated, one was found to be abundant in sewage (5.9×107 pfu/mL), having broad host range, and distinct protein and DNA profile when compared to the other six phages. DNA restriction and protein profiles of the phages and their morphology indicate the diversity in the sewage environment. None of the isolates from the rhizosphere regions of various cultivated soils were susceptible to phages isolated from sewage.
Keywords : Pseudomonas fluorescens; phages; RFLP; AFM; Protein analysis.