Abstract

SANTOS, Elisane Odriosolla dos et al. Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis. Braz. J. Microbiol. [online]. 2012, vol.43, n.2, pp. 432-440. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822012000200002.

The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2_v^5-1 fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).

Keywords : Raw glycerol; single cell protein; yeast biomass; Yarrowia lipolytica.