SciELO - Scientific Electronic Library Online

vol.43 issue2Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesisCharacterizations of a new Cordyceps cicadae isolate and production of adenosine and cordycepin author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Brazilian Journal of Microbiology

Print version ISSN 1517-8382


WEI, Shiping et al. Isolation and characterization of an Ashbya gossypii mutant for improved riboflavin production. Braz. J. Microbiol. [online]. 2012, vol.43, n.2, pp.441-448. ISSN 1517-8382.

The use of the filamentous fungus, Ashbya gossypii, to improve riboflavin production at an industrial scale is described in this paper. A riboflavin overproducing strain was isolated by ultraviolet irradiation. Ten minutes after spore suspensions of A. gossypii were irradiated by ultraviolet light, a survival rate of 5.5% spores was observed, with 10% of the surviving spores giving rise to riboflavin-overproducing mutants. At this time point, a stable mutant of the wild strain was isolated. Riboflavin production of the mutant was two fold higher than that of the wild strain in flask culture. When the mutant was growing on the optimized medium, maximum riboflavin production could reach 6.38 g/l. It has even greater promise to increase its riboflavin production through dynamic analysis of its growth phase parameters, and riboflavin production could reach 8.12 g/l with pH was adjusted to the range of 6.0-7.0 using KH2PO4 in the later growth phase. This mutant has the potential to be used for industrial scale riboflavin production.

Keywords : Riboflavin; Ashbya gossypii; Ultraviolet irradiation; Medium optimization.

        · text in English     · English ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License