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Brazilian Journal of Microbiology
Print version ISSN 1517-8382
HUASAI, Simujide et al. Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China. Braz. J. Microbiol. [online]. 2012, vol.43, n.2, pp.528-534. ISSN 1517-8382. http://dx.doi.org/10.1590/S1517-83822012000200013.
Virulence genes of Escherichia coli (E. coli) isolates from healthy dairy cows were identified and characterized by a multiplex PCR assay and serogrouping test. The results showed that among the target genes, eaeA was most frequently detected, accounting for 22.11% (67/303) in all strains from 101 cows. For categorization of E. coli, aEPEC was the category with widest distribution detected in 55 (18.15%) strains from 22 cattle. All of 84 PCR-positive strains belonged to 14 O serogroups, and O149 (25.00%) was most common identified, followed by O2 (17.86%), O8 (11.90%) and O103 (9.52%) with relatively high prevalence.
Keywords : E. coli; Virulence gene; Multiplex PCR; Serogroup; Healthy dairy cow.