Abstract

QIU, Shanlian; CHEN, Jichen; LIN, Si  and  LIN, Xinjian. A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel. Braz. J. Microbiol. [online]. 2012, vol.43, n.2, pp. 649-652. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822012000200029.

Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.

Keywords : Backed gel; DNA; Silver staining.