Services on Demand
- Cited by SciELO
- Access statistics
- Cited by Google
- Similars in SciELO
- Similars in Google
Brazilian Journal of Microbiology
Print version ISSN 1517-8382
TIRUMALAI, Prem Saran and PRAKASH, Soam. Expression of virulence genes by Listeria monocytogenes J0161 in natural environment. Braz. J. Microbiol. [online]. 2012, vol.43, n.2, pp. 834-843. ISSN 1517-8382. http://dx.doi.org/10.1590/S1517-83822012000200050.
Majority of studies concerning the gene expression of Listeria monocytogenes have been done on pure culture states. Our objective was to study L.monocytogenes in a co-cultured state and to understand if microbes in their natural state of existence are different in their expression than that of the purely cultured lab grown forms. For a long period discussions have been on the expression of prfA, (which is a virulence gene regulator) in a mammalian host and its role in causing the switch from a saprophytic to pathogenic form of L.monocytogenes. We, in this paper for the first time report the expression of prfA and other virulence genes by L.monocytogenes under different extracellular conditions, and also as a pure culture biofilms, that is different from the previous reports. We also report that the expression of prfA seems to vary considerably when co-cultured with Bacillus subtilis.
Keywords : Listeria monocytogenes; prfA; Virulence; Microarray; Biofilms; Co-culture; Gene Expression.