SciELO - Scientific Electronic Library Online

 
vol.45 issue2Canthaxanthin biosynthesis by Dietzia natronolimnaea HS-1: effects of inoculation and aeration rateImprovement of yield of the edible and medicinal mushroom Lentinula edodes on wheat straw by use of supplemented spawn author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Brazilian Journal of Microbiology

Print version ISSN 1517-8382

Abstract

IRFAN, Muhammad; NADEEM, Muhammad  and  SYED, Quratualain. Ethanol production from agricultural wastes using Sacchromyces cervisae. Braz. J. Microbiol. [online]. 2014, vol.45, n.2, pp.457-465. ISSN 1517-8382.  https://doi.org/10.1590/S1517-83822014000200012.

The main objective of this study was production of ethanol from three lignocellulosic biomasses like sugarcane bagasse, rice straw and wheat straw by Sacchromyces cervisae. All the three substrates were ground to powder form (2 mm) and pretreated with 3%H2O2 + 2% NaOH followed by steaming at 130 °C for 60 min. These substrates were hydrolyzed by commercial cellulase enzyme. The whole fermentation process was carried out in 500 mL Erlenmeyer flask under anaerobic conditions in submerged fermentation at 30 °C for three days of incubation period. FTIR analysis of the substrates indicated significant changes in the alteration of the structure occurred after pretreatment which leads to efficient saccharification. After pretreatment the substrates were hydrolyzed by commercial cellulase enzyme and maximum hydrolysis was observed in sugarcane bagasse (64%) followed by rice straw (40%) and wheat straw (34%). Among all these tested substrates, sugarcane bagasse (77 g/L) produced more ethanol as compared to rice straw (62 g/L) and wheat straw (44 g/L) using medium composition of (%) 0.25 (NH4)2SO4, 0.1 KH2PO4, 0.05 MgSO4, 0.25 Yeast extract by S. cervisae.

Keywords : lignoccellulosic biomass; Pretreatment; Sacchromyces cervisae; ethanol production.

        · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License