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Brazilian Journal of Microbiology

Print version ISSN 1517-8382

Abstract

NAVEED, Muhammad; AHMED, Iftikhar; KHALID, Nauman  and  MUMTAZ, Abdul Samad. Bioinformatics based structural characterization of glucose dehydrogenase (gdh) gene and growth promoting activity of Leclercia sp. QAU-66. Braz. J. Microbiol. [online]. 2014, vol.45, n.2, pp.603-611. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822014000200031.

Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p < 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains in GDH. GDH of Leclercia sp. QAU-66 is one of the main factor that involved in plant growth promotion and provides a solid background for further research in plant growth promoting activities.

Keywords : glucose dehydrogenase; pyrroloquinoline quinone; Leclercia sp; PGPR; bioinformatics.

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