A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
nitrile hydratase; amidase; nitrilase; amidase inhibitor; high-throughput screening
| Microorganism | Microbial control | Assay without DEPA | Assay with DEPA |
|---|---|---|---|
| Pseudomonas putida CCT 2357 | blue | yellow | yellow |
| Pseudomonas fluorescens CCT 3178 | blue | yellow | yellow |
| Arthrobacter sp CCT 1875 | yellow | yellow | yellow |
| Nocardia brasiliensis CCT 3439 | blue | blue | yellow |
| Nocardia simplex CCT 3022 | blue | yellow | greenish |
| Rhodococcus erythropolis CCT 1878 | blue | blue | yellow |
| Rhodococcus equi CCT 0541 | blue | blue | yellow |
| Rhodococcus ruber CCT 1879 | blue | blue | greenish |
