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Brazilian Journal of Microbiology

versão impressa ISSN 1517-8382versão On-line ISSN 1678-4405

Resumo

LI, Jiang; HU, Qiushi; LI, Yuquan  e  XU, Yuan. Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain. Braz. J. Microbiol. [online]. 2015, vol.46, n.3, pp.683-690.  Epub 21-Jul-2015. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-838246320131289.

An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg2+, Mn2+, K+; Ca2+, Na+, Ba2+, Zn2+, Cu2+, Co2+, Fe2+, Sr2+ and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.

Palavras-chave : psychrophilic bacteria; cold-adapted agarase; purification; characterization.

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