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Brazilian Journal of Microbiology

versão impressa ISSN 1517-8382versão On-line ISSN 1678-4405

Resumo

HAN, Xufeng et al. Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats. Braz. J. Microbiol. [online]. 2015, vol.46, n.3, pp.849-854. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-838246320140556.

The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum.

Palavras-chave : green fluorescent protein; gastro-intestinal tract; Lactobacillus plantarum; goat.

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