SciELO - Scientific Electronic Library Online

 
vol.46 número3Characterization of Francisella species isolated from the cooling water of an air conditioning systemMorphological changes and growth of filamentous fungi in the presence of high concentrations of PAHs índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Brazilian Journal of Microbiology

versão impressa ISSN 1517-8382versão On-line ISSN 1678-4405

Resumo

ALVAREZ-MEJIA, César et al. Characterization of the hrpZ gene from Pseudomonas syringae pv. maculicolaM2. Braz. J. Microbiol. [online]. 2015, vol.46, n.3, pp.929-936. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-838246320140655.

Pseudomonas syringae pv. maculicola is a natural pathogen of members of the Brassicaceae plant family. Using a transposon-based mutagenesis strategy in Pseudomonas syringaepv. maculicola M2 (PsmM2), we conducted a genetic screen to identify mutants that were capable of growing in M9 medium supplemented with a crude extract from the leaves of Arabidopsis thaliana. A mutant containing a transposon insertion in the hrpZ gene (PsmMut8) was unable to infect adult plants from Arabidopsis thaliana or Brassica oleracea, suggesting a loss of pathogenicity. The promotorless cat reporter present in the gene trap was expressed if PsmMut8 was grown in minimal medium (M9) supplemented with the leaf extract but not if grown in normal rich medium (KB). We conducted phylogenetic analysis using hrpAZB genes, showing the classical 5-clade distribution, and nucleotide diversity analysis, showing the putative position for selective pressure in this operon. Our results indicate that the hrpAZB operon from Pseudomonas syringaepv. maculicola M2 is necessary for its pathogenicity and that its diversity would be under host-mediated diversifying selection.

Palavras-chave : hrpZ; mutant non-pathogenic; transmid; Tn5; phylogenetic.

        · texto em Inglês     · Inglês ( pdf )