Chromatography |
Gelatinolytic |
X |
BAE (trypsin-like), Elastin, Synthetic Peptides |
0.088 nkat/mg |
X |
X |
X |
Ultrafiltration, Sephadex G-25 column |
Gelatinolytic |
X |
Casein, Elastin, Synthetic Peptides |
X |
EDTA, DFP, TLCK, TPCK |
X |
X |
Ammonium sulfate |
Collagenolytic |
X |
Casein, Elastin, Collagen, Gelatin, p-nitrophenol caprylate |
3.6 U/mg |
Ca2+, Na+, EDTA, 2,4-DNP |
pH 7, 35º |
X |
X |
Collagenolytic |
X |
BAPA, TAME |
X |
PMSF, TPCK, IAA2-mercaptoethanol, cysteine HCl, Zn, Ca, Mg, EDTA, Ca, Mg |
pH 8.4, 60º |
X |
(NH4)2SO4 Ammonium Sulfate, Sephadex G25, Biogel A |
Collagenolytic |
X |
Azocasein, Type I Collagen, Elastin |
X |
EDTA, fenantrolin, PA, PMSF, elastina, NEM |
X |
X |
Cation exchange chromatography |
Collagenolytic |
X |
Casein, Elastin, Orange Collagen |
0.39 U/mg |
X |
X |
X |
GF, Orange 3, Yellow 1, HA, TSK |
Collagenolytic |
VFLGREPKPDAFY |
Synthetic Peptides, Collagen |
X |
Fenantrolina, EDTA, |
X |
X |
X |
Collagenolytic |
X |
Synthetic Collagen, Peptides |
70.4 U/mg |
X |
pH 8.2 |
X |
Ammonium sulfate and cation exchange chromatography |
X |
AEQTDSTWGL |
Casein, BSA, Skimmed milk, Gelatin, Collagen, Denatured Collagen, Nematode cuticle |
0.37 U/mg |
PMSF, EDTA, Pepstatin A, Leupeptin, Aprotinins |
pH 10, 55º |
X |
Ammonium sulfate, Q Sepharose FF, Sephacryl S-100 |
Gellatonolytic |
X |
Casein, Gelatin, Nematode cuticle, Azocoll |
1.12 U/mg |
PMSF e SSI |
pH 6-8, 45º |
4.9 |
Ultrafiltration, HITrap SP FF, HiPrep phenyl FF |
Gellatonolytic |
AITQQQGAPW |
Casein, BSA, Gelatin, Collagen, Nematode cuticle |
48 U/mg |
Leupeptin, Aprotinin, EDTA, Pepstatin A, PMSF |
pH 10, 70º |
X |
Source 15Q, Phenyl Superose |
Gellatonolytic |
AEQLDSTWGL |
Casein, BSA, Skimmed milk, Gelatin, Hydrolyzed Collagen |
X |
PMSF |
pH 9, 60º |
6.8 |
Ammonium Sulfate, Sephadex G-25 e DEAE-cellulose |
Collagenolytic |
X |
X |
92.17 U/mg |
Cetrimide |
X |
X |
X |
Gelatinolytic |
X |
Keratin, Elastase, Synthetic Peptide |
X |
X |
X |
X |
Ammonium sulfate, DEAE-cellulose, Sephadex G150 |
Collagenolytic |
X |
Collagen, Casein, elastin |
18,064.7x103 U/mg |
EDTA, Iodoacetate, Sodium arsenate, arsenito, Cysteine |
pH 5, 40º |
X |
X |
X |
X |
Casein, Gelatin, Keratin, Albumin, Hemoglobin |
X |
PMSF |
X |
X |
X |
Collagenolytic |
X |
Casein, Elastase. Azocoll |
X |
PMSF, EDTA, Phenanthroline |
X |
X |
X |
Collagenolytic |
X |
Azocoll, Type I collagen, Gelatin, Azocasein |
319 U/mg |
PMSF, iodoacetic acid, EDTA e Pepstatin A |
pH 9, 37º |
X |
X |
Collagenolytic |
X |
X |
X |
X |
X |
X |
X |
Collagenolytic |
X |
Casein, Keratin |
X |
PMSF, EDTA, IAA |
pH 6.5, 55º |
X |
X |
Collagenolytic |
X |
Plasmin, Plasminogen, Azocoll |
X |
X |
X |
X |
Purif. |
Molecular weight (kDa) |
Col. activ. |
Col. activ. method. |
Culture conditions |
Culture medium |
Microorg. |
Authors |
Chromatography |
23-40 |
X |
Synthetic peptide |
pH 5.6, 30º C, without agitation, 15 days |
Casamino acids, Dextrose, CaCl2, YE and Berthelot solution |
E. coronata
|
Hurion et al. (1977)4141 Hurion N, Fromentin H, Keil B. Proteolytic enzymes of Entomophthora coronata. Characterization of a collagenase. Comp Biochem Physiol. 1977;56:259-264.
|
Ultrafiltration, Sephadex G-25 column |
X |
X |
Synthetic peptide |
pH 5.6, 30º C, without, 15 days |
Casamino acids, Dextrose, CaCl2, YE and Berthelot solution |
E. coronata
|
Hurion et al. (1979)4242 Hurion N, Fromentin H, Keil B. Specificity of the collagenolytic enzyme from the fungus Entomophthora coronata: comparison with the bacterial collagenase from Achromobacter iophagus. Arch Biochem Biophys. 1979;192(2):438-445.
|
Ammonium sulfate |
X |
X |
Achilles tendon bovine |
7 days, 30 ºC |
Glucose, salts, l-cysteine, tryptone, biotin, thiamine |
A. aculeatus
|
Olutiola and Nwaogwugwu (1982)4343 Olutiola PO, Nwaogwugwu RI. Growth, sporulation and production of maltase and proteolytic enzymes in Aspergillus aculeatus. Trans Br Mycol Soc. 1982;78(1):105-113.
|
X |
X |
8 U/mL |
Azocoll |
Gyrotory shaker (20-24 ºC), sob luzes fluorescents |
Peptone, yeast extract and glucose (PYG) broth |
L. giganteum
|
Dean and Domnas (1983)4444 Dean DD, Domnas AJ. The extracellular proteolytic enzymes of the mosquito-parasitizing fungus Lagenidium giganteum. Exp Mycol. 1983;7(1):31-39.
|
(NH4)2SO4 Ammonium Sulfate, Sephadex G25, Biogel A |
X |
X |
SDS-PAGE |
2 weeks, T.A. |
M9 (without NH4Cl) + collagen |
A. flavus
|
Zhu et al. (1990)4545 Zhu WS, Wojdyla K, Donlon K, Thomas PA, Eberle HI. Extracellular proteases of Aspergillus flavus. Fungal keratitis, proteases, and pathogenesis. Diagn Microbiol Infect Dis. 1990;13:491-497.
|
Cation exchange chromatography |
32 |
X |
Orange collagen e Synthetic Peptide |
5 days, 37 ºC, 150 rpm |
Yeast carbon base + collagen type I |
Aspergillus
|
Tomee et al. (1994)4646 Tomee JF, Kauffman HF, Klimp AH, de Monchy JG, Köeter GH, Dubois a E. Immunologic significance of a collagen-derived culture filtrate containing proteolytic activity in Aspergillus-related diseases. J Allergy Clin Immunol. 1994;93(4):768-778.
|
GF, Orange 3, Yellow 1, HA, TSK |
82 |
X |
Rat native type I collagen |
25 ºC, pH 6, 7 days |
Sabouraud |
T. schoenleinii
|
Ibrahim-Granet et al. (1996)4747 Ibrahim-Granet O, Hernandez FH, Chevrier G, Dupont B. Expression of PZ-peptidases by cultures of several pathogenic fungi. Purification and characterization of a collagenase from Trichophyton schoenleinii. J Med Vet Mycol. 1996;34(2):83-90.
|
X |
X |
70.4 U/mL |
Ninhydrin |
pH 7, 25 ºC, 50 h, with agitation |
YPG |
Z. rouxii
|
Ok and Hashinaga (1996)4848 Ok T, Hashinaga F. Detection and production of extracellular collagenolytic enzyme from Zygosaccharomyces rouxii. J Gen Appl Microbiol. 1996;42:517-523.
|
Ammonium sulfate and cation exchange chromatography |
35 |
1% and 2% |
Azocoll |
26 ºC, 200 rpm, 7 days |
LMZ |
P. chrysogenum
|
Benito et al. (2002)4949 Benito MJ, Rodríguez M, Núñez F, Miguel a, Bermúdez ME, Córdoba JJ. Purification and characterization of an extracellular protease from Penicillium chrysogenum Pg222 active against meat proteins purification and characterization of an extracellular protease from Penicillium chrysogenum Pg222 active against meat proteins. 2002;68(7):5–10.
|
Ammonium sulfate, Q Sepharose FF, Sephacryl S-100 |
38 |
0.0134 U/mL/m |
Azocoll |
25-18 ºC, 6 days,150-200 rpm pH 6.5 |
LMZ - with gelatin |
A. oligospora
|
Minglian et al. (2004)5050 Minglian Z, Minghe M, Keqin Z. Characterization of a neutral serine protease and its full-length cDNA from the nematode-trapping fungus Arthrobotrys oligospora. Mycologia. 2004;96(1):16-22.
|
Ultrafiltration, HITrap SP FF, HiPrep phenyl FF |
32 |
14% |
Non described |
26 ºC, 100 rpm, 6 days |
Glucose, gelatin and salts |
L. psalliotae
|
Yang et al. (2005)5151 Yang J, Huang X, Tian B, Wang M, Niu Q, Zhang K. Isolation and characterization of a serine protease from the nematophagous fungus. Lecanicillium psalliotae, displaying nematicidal activity. Biotechnol Lett. 2005;27(15):1123-1128.
|
Source 15Q, Phenyl Superose |
39 kDa |
Collagen 15.9%, Denatured Collagen 48.1%, |
Folin |
6 days, 26 ºC, 200 rpm |
Protease inducing - with gelatin |
M. microscaphoides
|
Wang et al. (2006)5252 Wang M, Yang J, Zhang K-Q. Characterization of an extracellular protease and its cDNA from the nematode-trapping fungus Monacrosporium microscaphoides. Can J Microbiol. 2006;52(2):130-139.
|
Ammonium Sulfate, Sephadex G-25 e DEAE-cellulose |
72-92 kDa |
82.95 U/mL |
Ninhydrin |
6 days, 37 ºC |
Gelatin, glucose, yeast extract, and native bovine collagen |
A. flavus
|
Mahmoud et al. (2007)5353 Mahmoud Y-G, Abu El-Souod SM, El-Shourbagy SM, El-Badry ASM. Characterisation and inhibition effect of cetrimide on collagenase produced byAspergillus flavus, isolated from mycotic ulcers. Ann Microbiol. 2007;57(1):109-113.
|
X |
X |
1 Unit of collagenase |
Synthetic peptide |
14 days |
Medium with type I collagen |
M. canis
|
Viani et al. (2007)5454 Viani FC, Cazares Viani PR, Gutierrez Rivera IN, da Silva ÉG, Paula CR, Gambale W. Actividad proteolítica extracelular y análisis molecular de cepas de Microsporum canis aisladas de gatos con y sin sintomatología. Rev Iberoam Micol. 2007;24(1):19-23.
|
Ammonium sulfate, DEAE-cellulose, Sephadex G150 |
66 kDa |
212.33 U/mL |
Ninhydrin |
108 h, 175 rpm, pH 5.5, 30 ºC |
Sabouraud-glucose-collagen |
R. solani
|
Hamdy (2008)5555 Hamdy HS. Extracellular collagenase from Rhizoctonia solani: production, purification and characterization. Indian J Biotechnol. 2008;7(July):333-340.
|
X |
25 kDa |
X |
Zymogram |
pH 5.5, 9 days, T.A. |
Czapek |
C. immitis
|
Lopes et al. (2008)5656 Lopes BGB, Santos LSD, Bezerra CDCF, et al. A 25-kDa serine peptidase with keratinolytic activity secreted by Coccidioides immitis. Mycopathologia. 2008;166(1):35-40.
|
X |
20-200 kDa |
1.2 U/mL |
Azocoll |
150 rpm, 35 ºC, for 7, 14, 21 e 28 days |
Yeast carbon base + collagen + vitamin solution; neopeptoneBHI + elastin |
P. brasiliensis
|
Voltan et al. (2008)5757 Voltan AR, Donofrio F, Miranda ET, Moraes RA, Mendes-Giannini MJS. Induction and secretion of elastinolytic and proteolytic activity in cultures of Paracoccidioides brasiliensis. Rev Ciências Farm Básica e Apl. 2008;29(1):97-106.
|
X |
X |
164 U/mL |
Azocoll |
0.75% gelatin, 200 rpm, pH 8.0 and 28 ºC |
Soybean flour, glucose and mineral solution |
P. aurantiogriseum
|
Lima et al. (2011a)2424 Lima CA, Filho JLL, Neto BB, Converti A, Carneiro da Cunha MG, Porto ALF. Production and characterization of a collagenolytic serine proteinase by Penicillium aurantiogriseum URM 4622: a factorial study. Biotechnol Bioprocess Eng. 2011;16(3):549-560.
|
X |
X |
231 U/mL |
Azocoll |
pH 7.0, 24 ºC, 24 h |
Soybean flour, Glucose and mineral solution |
P. aurantiogriseum
|
Lima et al. (2011b)5858 Lima CA, Viana Marques DA, Neto BB, Lima Filho JL, Carneiro-da-Cunha MG, Porto ALF. Fermentation medium for collagenase production by Penicillium aurantiogriseum URM4622. Biotechnol Prog. 2011;27(5):1470-1477.
|
X |
X |
0.165 OD/mL |
Azocoll |
2.0 × 105 espores, 72 h, 30 ºC, 75% humidity |
Solid medium of wheat bran |
A. terreus
|
de Siqueira et al. (2014)5959 de Siqueira ACR, da Rosa NG, Motta CMS, Cabral H. Peptidase with keratinolytic activity secreted by Aspergillus terreus during solid-state fermentation. Braz Arch Biol Technol. 2014;57(4):514-522.
|
X |
X |
113.2 and 332 × 10-3 U/mL |
Azocoll |
200 rpm, 28 ºC, 4 days |
Several |
Micromycetes
|
Sharkova et al. (2015)2626 Sharkova TS, Kurakov AV, Osmolovskiy AA, et al. Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes. Microbiology. 2015;84(3):359-364.
|