Growth kinetic models of five species of <i>Lactobacilli</i> and lactose consumption in batch submerged culture

Rezvani, Fazlollah; Ardestani, Fatemeh; Najafpour, Ghasem

doi:10.1016/j.bjm.2016.12.007

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Brazilian Journal of Microbiology

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Industrial Microbiology, Research Paper • Braz. J. Microbiol. 48 (2) • Apr-Jun 2017 • https://doi.org/10.1016/j.bjm.2016.12.007 copy

Growth kinetic models of five species of Lactobacilli and lactose consumption in batch submerged culture

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Kinetic behaviors of five Lactobacillus strains were investigated with Contois and Exponential models. Awareness of kinetic behavior of microorganisms is essential for their industrial process design and scale up. The consistency of experimental data was evaluated using Excel software. L. bulgaricus was introduced as the most efficient strain with the highest biomass and lactic acid yield of 0.119 and 0.602 g g^-1 consumed lactose, respectively. The biomass and carbohydrate yield of L. fermentum and L. lactis were slightly less and close to L. bulgaricus. Biomass and lactic acid production yield of 0.117 and 0.358 for L. fermentum and 0.114 and 0.437 g g^-1 for L.actobacillus lactis were obtained. L. casei and L. delbrueckii had the less biomass yield, nearly 11.8 and 22.7% less than L. bulgaricus, respectively. L. bulgaricus (R ² = 0.9500 and 0.9156) and L. casei (R ² = 0.9552 and 0.8401) showed acceptable consistency with both models. The investigation revealed that the above mentioned models are not suitable to describe the kinetic behavior of L. fermentum (R ² = 0.9367 and 0.6991), L. delbrueckii (R ² = 0.9493 and 0.7724) and L. lactis (R ² = 0.8730 and 0.6451). Contois rate equation is a suitable model to describe the kinetic of Lactobacilli. Specific cell growth rate for L. bulgaricus, L. casei, L. fermentum, L. delbrueckii and L. lactis with Contois model in order 3.2, 3.9, 67.6, 10.4 and 9.8-fold of Exponential model.

Keywords:
Bacterial growth; Exponential kinetic model; Contois model; Lactobacilli

		Time (h)
		0	6	12	18	24	30	36	42	48	52
L. bulgaricus	X (g L^-1)	0	0.2	0.9	2.5	4.2	5.1	5.1	5	5.1	5.2
	S (g L^-1)	50	47.9	40.1	26.6	13.5	7.3	7	6.7	6.1	5.8
	µ (h^-1)	-	0	0.250	0.210	0.169	0.135	0.108	0.089	0.077	0.071
L. casei	X (g L^-1)	0	0.2	0.8	2.6	3.9	4.2	4.3	4.3	4.2	3.8
	S (g L^-1)	50	48.2	42.1	31.9	17.8	10.5	9.2	9.1	8.7	8
	µ (h^-1)	-	0	0.231	0.214	0.165	0.127	0.102	0.085	0.072	0.064
L. lactis	X (g L^-1)	0	0.2	0.6	1.9	3.1	3.2	3.1	3.4	3.9	3.1
	S (g L^-1)	50	49.2	38.8	27.3	21.4	19.6	18.2	16.4	15.8	13.2
	µ (h^-1)	-	0	0.183	0.188	0.152	0.115	0.091	0.079	0.071	0.059
L. delbrueckii	X (g L^-1)	0	0.2	0.6	1.7	2.4	2.6	2.9	3.2	3.1	3
	S (g L^-1)	50	48.1	36.4	25.3	22.1	18.2	16.4	15.1	13.6	12.4
	µ (h^-1)	-	0	0.183	0.178	0.138	0.107	0.089	0.077	0.065	0.058
L. fermentum	X (g L^-1)	0	0.1	0.7	2.1	2.5	2.7	2.7	2.9	3.3	3.5
	S (g L^-1)	50	49.3	44.2	35.6	29.1	26.8	25.3	23.2	22.6	20.1
	µ (h^-1)	-	0	0.324	0.254	0.179	0.137	0.110	0.093	0.083	0.077

Strain	R ²		µ_max (h^-1)		K_s (g L^-1)
	Contois	Exponential	Contois	Exponential	Contois
L. delbrueckii subsp. bulgaricus PTCC1737	0.9500	0.9156	0.265	0.0824	1.34
L. casei subsp. casei PTCC1608	0.9552	0.8401	0.299	0.0757	3.58
L. delbrueckii subsp. lactis PTCC 1743	0.8730	0.6451	0.333	0.0339	12.72
L. delbrueckii subsp. delbrueckii PTCC1333	0.9493	0.7724	0.311	0.03	13.11
L. fermentum PTCC1744	0.9367	0.6991	2.591	0.0383	188.33

Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br

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[1] *Corresponding author. E-mail: f.ardestani@qaemiau.ac.ir Ardestani_fatemeh@yahoo.com (F. Ardestani).